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To investigate the effect of Trypsin using a photographic film
- https://blablawriting.net/prime-investigate-effect-trypsin-using-photographic-film-essay
- Preliminary experiment: We are investigating the best pH at which Trypsin works. Method: A beaker was filled with water of about 400C. Each test tube was filled with 5cm3 of trypsin and 5cm3 of water with a buffer solution one pH 4 one pH 7 and the other pH 9. A piece of 1cm2 photographic film was dropped in. At this time the stop clock was started.
Investigating effect of concentration on the activity of …
- https://practicalbiology.org/bio-molecules/factors-affecting-enzyme-activity/investigating-effect-of-concentration-on-the-activity-of-trypsin
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To investigate the effect of Trypsin using a photographic film
- https://www.markedbyteachers.com/as-and-a-level/science/to-investigate-the-effect-of-trypsin-using-a-photographic-film.html
- A piece of the film was placed in each test tube. At this time a stop clock is started. Each test tube is monitored until the film goes clear. Results table: Analysis: The optimum pH of the enzyme was pH 9, which is what I predicted the preliminary experiment showed this.
Determining the Optimum pH for Trypsin - Biocyclopedia
- https://biocyclopedia.com/index/biotechnology_methods/enzymology/determining_the_optimum_ph_for_trypsin.php
- In this experiment, we place a photographic film strip into a trypsin solution. The protease will digest the gelatin coating and make it whitish. Different buffer solutions will be added to the solutions as well, to change the surrounding pH. The degree of digestion of the gelatin reflects the activity of the enzyme.
To investigate the effect of temperature on the rate of reaction on ...
- https://blablawriting.net/prime-investigate-effect-temperature-rate-reaction-trypsin-enzymes-essay
- 7. Carefully place a 1cm piece of photographic film in each notch on the splints. 8. Place three test tubes in the water bath. 9. Set the thermostat on the water bath to 10C. 10. Wait 10-12 minutes for the water to settle at 10C. 11. Place a splint with photographic film into each of the test tubes. 12. Start timing using the stopwatch. 13.
Procedure - Royal Society of Biology
- https://practicalbiology.org/images/pbiol/doc/migrated/investigating-effect-of-concentration-on-the-activity-of-trypsin-ss-33__1_.docx
- Cut pieces of photographic film to fit in the test tube as shown. (This may have been done for you.) Attach wooden spills to each piece of film to be used (see diagram), or tie a piece of cotton thread through a sprocket hole and suspend the film from a spill placed across the tube.
Investigating The Rate Of Reaction Of Enzymes In The Clearing Of ...
- https://essaymania.com/50508/investigating-the-rate-of-reaction-of-enzymes-in-the-clearing-of-photographic-film
- In the investigation I am going to study one of the four factors that alter the rate of reaction of trypsin in the clearing of photographic film. In photographic film is the clear acetate at the bottom and on top of that is geletin (a substrate) which has silver salts in it. When trypsin is added, the geletin is broken down and the acetate becomes clear. This can occur at different …
A spectrophotometric determination of trypsin and
- https://www.sciencedirect.com/science/article/pii/0006300255902808
- The extinction coefficient used for chymotrypsin is 2.o75/cm/mg/mlla and that for trypsin 1.7I/cm/mg/m114. RESULTS The difference spectra of o.ool M solutions of L-tyrosine at various pH values with reference to a o.ooi M solution of L-tyrosine at pH 3.o7 are shown in the lower part of Fig. I. The effect of dissociable groups other than the ...
Biology Coursework: The effect of Trypsin on Gelatine. | 123 Help …
- https://www.123helpme.com/essay/Biology-Coursework-The-effect-of-Trypsin-on-55569
- § Photographic film (b/w) § Syringe § Test tube rack § Ruler § Scissors § O.1% trypsin made in pH7 buffer solution § Mounted needle 2. I then decided on the range of results and temperatures I was to investigate. I decided to observe 20º, 30º, 40º, 50º, 60º, 70º and 80º. 3.
pH effects on the reaction rates of Trypsin by Ehsaan …
- https://prezi.com/0f3kfkj8ha5l/ph-effects-on-the-reaction-rates-of-trypsin/
- Basic Method. Fill 6 test tubes with 2% trypsin (A-F) and a different pH buffer solution (4-11) (Experimental) Heat water bath to 38 degrees, place in test tubes, then place a wooden splint with film attached into each test tube. Start to …
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